There is no particular sequence for Apps Tier nodes.
When running Autoconfig in EBS environment start Autoconfig with Database Tier node and then on admin/primary node and then continue with other nodes
Hope this answers your question
1- autoconfig -> 2 node WEB -> the 2 node CM -> 2 node DMZ -> first node WEB
before running autoconfig take the backup files which modified for url and port mask while configuring the DMZ
Thanks for the update.
When we run autoconfig, values will change in DMZ nodes?
Every time, manually Need to change to original value?
Could you please provide details about the values that you found / assume as changing in DMZ nodes
What I have found is if you run autoconfig on the DMZ tier, it always sets the site level profile options for the %AGENT% profiles to the DMZ host and internal users can no longer login. You should run autoconfig on the primary internal tier last on 12.1.